RBS

Part:BBa_K3453005:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-03)


Synthetic RBS stem-loop designed for standard toehold switches


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically designed by Pardee et al. [1] to be part of toehold switches.

Source

DNA synthesis

References

[1] Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM, O'Connor DH, Gehrke L, Collins JJ. Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell (2016) 165, 1255-1266.